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KMID : 0613820060160071225
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Journal of Life Science
2006 Volume.16 No. 7 p.1225 ~ p.1228
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Mutant cAMP Receptor Protein Binds to DNA without DNA Bending
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Gang Jong-Back
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Abstract
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Cyclic AMP receptor protein (CRP) complexed with cAMP binds to DNA and induces sharp DNA bending around ~90 degree. Previous publication [5], however, reported that mutant CRP:cGMP complex showed high migration rate relative to mutant CRP:cAMP complex on native polyacrylamide gel. To confirm DNA structural change in the presence of CRP and cyclic nucleotide, molar cyclization factor (jM) [13] was measured with 6 constructed DNA fragments. Nonlinear regression analysis of jM data indicated that mutant CRP did not induce DNA bending in the presence of cGMP but bent DNA in the presence of cAMP without any helical twist change in DNA.
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KEYWORD
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Cyclic AMP receptor protein, molar cyclization factor(jM), DNA structural change
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